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MPs have voted not to give their approval to the prime minister's Brexit deal until he has passed all necessary legislation.
Cambridge MP Daniel Zeichner (Labour) voted for the amendment as did South Cambridgeshire's Heidi Allen (Lib Dem).
Stephen Barclay (Conservative) voted against as did Lucy Frazer for South East Cambridgeshire, West Suffolk MP Matt Hancock (Conservative) and Kemi Badenoch (Conservative)
The result will effectively force Boris Johnson to seek an extension to the Article 50 negotiating period tonight, possibly delaying Brexit beyond 31 October.
The House of Commons voted 322 to 306 in favour of an amendment, proposed by former Conservative minister Sir Oliver Letwin, to a motion on the prime minister's Brexit deal.
The vote went against Mr Johnson, who has promised to deliver Brexit on 31 October "do or die", after the DUP gave their backing to Sir Oliver's amendment.
The Northern Ireland party are fiercely opposed to the prime minister's Brexit deal.
WHAT HAPPENS NOW?
- The PM is compelled to write to the EU requesting an Article 50 extension beyond 31 October
- He will attempt to put his Brexit deal to another vote in the House of Commons on Monday
- The government will also next week introduce necessary Brexit legislation
- If the legislation passes before 31 October, the UK is still able to leave the EU on that date